rapid gel filtration (Bio-Rad)
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rapid gel filtration
Rapid Gel Filtration, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 4041 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rapid+gel+filtration/Gel+Filtration+Standard/10__7554_slash_elife__67732-346-12-18
Average 96 stars, based on 4041 article reviews
Rapid Gel Filtration, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 4041 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rapid+gel+filtration/Gel+Filtration+Standard/10__7554_slash_elife__67732-346-12-18
Average 96 stars, based on 4041 article reviews
rapid gel filtration - by Bioz Stars,
2026-09
96/100 stars
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Filtration:Article Title: A single power stroke by ATP binding drives substrate translocation in a heterodimeric ABC transporter Article Snippet: 200 nM TmrAB reconstituted in lipid nanodiscs were incubated with 2.8 mM ATP supplemented with 0.2 mM of [2,5’,8-3H(N)]-ATP (3H-ATP, PerkinElmer) or 2.8 mM ADP supplemented with 0.2 mM of [2,8-3H]-ADP (3H-ADP, Biotrend), and 3 mM MgCl2. .. If indicated, the excess of free nucleotides was removed by Article Title: De novo macrocyclic peptides dissect energy coupling of a heterodimeric ABC transporter by multimode allosteric inhibition Article Snippet: Detergent-solubilized TmrAB (2 mM) were incubated with ATP (1 mM) traced with [a32P]-ATP (Hartmann Analytic), MgCl2 (5 mM), CP Fs (4 mM), or orthovanadate (1 mM) for 5 min at 45 ̊C. .. Cold ATP (10 mM) was added, and unbound nucleotides were removed by Article Title: A single power stroke by ATP binding drives substrate translocation in a heterodimeric ABC transporter Article Snippet: In brief, TmrA EQ B (2 μM) reconstituted in lipid nanodiscs was incubated with 3 mM MgCl 2 and 1 mM ATP traced with [γ 32 P]-ATP or [α 32 P]-ATP (Hartmann Analytic) for 5 min at 45°C. .. After arresting TmrA EQ B in the OF conformation, 2 mM of cold ATP were added and free radiolabeled nucleotides were removed by Article Title: De novo macrocyclic peptides dissect energy coupling of a heterodimeric ABC transporter by multimode allosteric inhibition Article Snippet: Detergent-solubilized TmrAB (2 μM) were incubated with ATP (1 mM) traced with [α 32 P]-ATP (Hartmann Analytic), MgCl 2 (5 mM), CP F s (4 μM), or orthovanadate (1 mM) for 5 min at 45°C. .. Cold ATP (10 mM) was added, and unbound nucleotides were removed by Article Title: A single power stroke by ATP binding drives substrate translocation in a heterodimeric ABC transporter Article Snippet: 200 nM TmrAB reconstituted in lipid nanodiscs were incubated with 2.8 μM ATP supplemented with 0.2 μM of [2,5’,8- 3 H(N)]-ATP ( 3 H-ATP, PerkinElmer) or 2.8 μM ADP supplemented with 0.2 μM of [2,8- 3 H]-ADP ( 3 H-ADP, Biotrend), and 3 mM MgCl 2 . .. If indicated, the excess of free nucleotides was removed by Article Title: A single power stroke by ATP binding drives substrate translocation in a heterodimeric ABC transporter Article Snippet: To induce the IF-to-OF transition, TmrA EQ B was incubated with 1 mM ATP (Sigma-Aldrich) and 3 mM MgCl 2 for 5 min at 45 °C. .. If indicated, the excess of free ATP and peptide was removed by Article Title: A single power stroke by ATP binding drives substrate translocation in a heterodimeric ABC transporter Article Snippet: In brief, TmrAEQB (2 mM) reconstituted in lipid nanodiscs was incubated with 3 mM MgCl2 and 1 mM ATP traced with [g 32P]-ATP or [a32P]-ATP (Hartmann Analytic) for 5 min at 45 ̊C. .. After arresting TmrAEQB in the OF conformation, 2 mM of cold ATP were added and free radiolabeled nucleotides were removed by other:Article Title: De novo macrocyclic peptides dissect energy coupling of a heterodimeric ABC transporter by multimode allosteric inhibition Article Snippet: ATP (10 mM) was added, and samples were spotted onto polyethyleneimine cellulose plates (Merck Millipore). |